Biomedically assisted fertilization procedures are methods that control and assist not only the fertilization of an egg but also the transfer of gametes, implantation, and the development of pregnancy. In Best IVF Center in Pakistan is the basic method of biomedically assisted fertilization, which bypasses the fallopian tubes and combines male and female gametes outside the body. It is performed when all other infertility treatment options have been exhausted, or when surgery, drug treatment, or combined treatment is not possible or not successful.
FERTILIZATION PROCEDURES WITH BIOMEDICAL ASSISTANCE
Introduction of the partner’s sperm into the uterus – intrauterine insemination (IUI),
Introduction of donor sperm into the uterus (AID),
Freezing and thawing of embryos (cryopreservation ),
Direct introduction of sperm into the oocyte – (ICSI),
Direct introduction of selected sperm into the oocyte – (IMSI).
We can help with both female and Male Infertility Clinic in Lahore with biomedically assisted fertilization procedures. Female causes include: endometriosis, blocked fallopian tubes, ovulation disorders (e.g. polycystic ovary syndrome unresponsive to drug treatment), chromosomal abnormalities, and unexplained infertility. Men can be helped in the case of poor quality semen (oligo-, asthenic-, teratozoospermia), when the semen is without sperm (azoospermia), with chromosomal (genetic) disorders and unexplained infertility.
Stimulation of the ovaries with hormones
The process of in vitro fertilization begins by stimulating the ovaries with pituitary hormones (gonadotropins) through daily injections. With gonadotropin hormones, we achieve the growth and development of several follicles at the same time and the maturation of several oocytes (unlike the spontaneous cycle, when a single cell grows and matures).
There are several different protocols for ovarian stimulation. The dose of hormones and the type of protocol are determined by the gynecologist who takes care of the in vitro fertilization procedure, taking into account previous findings and procedures. Excessive dosage of hormones can provoke hyperstimulation (in 2-10% of women). This is more dangerous in women with polycystic ovary syndrome (PCOS) and in young, thin women.
Ultrasound monitoring of follicle growth and 3. oocyte maturation – stop injection
With regular vaginal ultrasound examinations, we determine the number and growth of the follicles in which the egg cells grow, and evaluate the thickness of the uterine mucosa. When the growing follicles reach the optimal size, the woman receives a ” stop injection ” that causes the final maturation of the oocytes.
Ultrasound-guided ovarian puncture & sperm release
On the second day after the “stop injection”, follicle aspiration is performed, with which the oocytes are obtained. Your partner releases semen on this day.
The ICSI method, or the method of direct introduction of the sperm into the egg cell, is one of the most important methods for the treatment of male infertility. It is performed in men who have poor sperm quality (low sperm count, poorly motility sperm, sperm of a changed shape, or even sperm found in the testicles of those men who have no sperm in the ejaculate). In this case, one sperm is inserted into each egg cell under a special inverted microscope in the biomedically assisted fertilization laboratory. Egg cells fertilized in this way are then incubated in the culture medium for growing egg cells in an incubator, just like other egg cells after classic IVF.
Embryo transfer to the uterus
If the cells are fertilized and develop correctly, a maximum of two embryos are transferred to the uterus at the same time. Since 2008, we have been transferring one good-quality embryo into the uterus in the first two biomedically assisted fertilization procedures in women up to 35 years of age. Embryo transfer can be done on the second, third or fifth day after ovarian puncture. Not all embryos develop with the same dynamics, but this is not decisive for the final result, since further divisions take place in the uterus.
If more than two embryos are obtained in the procedure, the supernumerary embryos can be frozen . The thawed embryos are later transferred to the uterus in a spontaneous or mildly stimulated cycle. This increases the possibility of pregnancy after one procedure or after one puncture.
Support of the luteal phase
The corpus luteum (or several corpus luteums after the process of stimulating ovulation) produces the hormone progestagen, which is necessary to prepare the lining of the uterus for the implantation of the embryo. After the puncture of several oocytes, the function of the corpus luteum is often insufficient, so by adding the hormone progesterone in the form of vaginalettes, we support the function of the corpus luteum. Progesterone is a hormone that maintains pregnancy and has no side effects on the fetus. IVF in Lahore begins the day after the puncture and continues for the next 14 days or until menstruation.